ü Transfection of DG44 and transformation of E. coli with GFP | UML | Summer ’11 to Present
- Optimized the protocol, transformation of plasmid containing GFP in E. coli and selection of transformed colonies. The favorable colonies were obtained in grown in broth cultures, and finally the plasmid DNA was isolated using DNA prep kit. The isolated DNA was then used for transfection of DG44 cells maintained in 30 shake flask. The transfected cells will be selected for stable expression by maintaining them under Genenticin (antibiotic) for a couple of weeks. The selected cells will then be cultured and cryopreserved for further use. The success criteria were determined by looking at the cell count, viability, and fluorescence images.
ü Compatibility study between Cedex Bio and other technologies in the market | UML | Summer ’11 to January ‘12
- Designed the experiment and constructed the protocol for comparing the quality of metabolic information obtained using the Cedex Bio and other existing membrane based technologies, and HPLC. 3 shake flasks and 2 bioreactor runs were run for 2 different cell lines, DG44 and FreeStyle CHO-S cells. The data was collected daily covering the full cell cycle. The data was analyzed and presented to the respective collaborators.
ü Multivariate data analysis of DG44 using NIR and Raman | UML | Spring ’11
- 5 to 6 bioreactor runs were performed using DG44 and FreeStyle CHO-S cells to collect data covering their full cell cycle. The pH, DO (dissolved oxygen), NIR, RF (radio frequency), cell count, metabolite information was collected each day. The collected data was organized, analyzed and documented.
ü Determining resin binding capacity using Raman spectra | UML | Spring ’11
ü Production of biodiesel using microalgae (Botryococcus braunii) | RVCE | 2009-2010
ü Production of bioethanol from lignocellulosic materials by biodegradation using P. chrysosporium | RVCE | Fall 2008
