ü Graduate Research Assistant | Massachusetts Bio-manufacturing Center (MBMC) | UML | February 2011 to Present
Cell culture: Culturing and maintaining CHO DG44, FreeStyle CHO-S, and Freedom CHO-S cells. Experienced in handling Cedex Bio, NOVA 400 (Both for metabolic information), Cedex HiRes (Cell count).
Bioreactor: Multivariate data collection and analysis of Applikon (AD 1010) and Sartorius systems (1 to 5 liters).
Molecular biology: Optimize protocol and perform transfection of DG44 with GFP, transformation of E. coli with plasmid DNA containing GFP.
Downstream: Protein titer analysis using HPLC, determining resin binding capacity using Raman spectra.
DOE (Design of Experiments): Basics with Minitab and JMP to create full factorial DOE’s.
Presentation: Weekly presentation of research work to Professors and fellow researchers and making valuable suggestions on other research issues.
ü Gradute Teaching Assistant | UML | September 2011 to Present
Conducted labs and recitations for undergraduate students enrolled in Unit Operations II and Introduction to Engineering course.
ü Student Intern | Center for Environment | Indian Institute of Technology (IIT) | Fall 2008
Cell culture: Maintenance and use of Arthrobacter chlorophenolicus for biodegradation of chlorophenol and paranitrophenol | Training: Blotting (Southern, Western) | Isolation of DNA (Genomic, Plasmid, Chloroplast) | Electrophoresis (Agarose, SDS-PAGE) | DNA Conjugation and Transformation | Chromatography (TLC, GC, HPLC) | PCR.
