ü Cell culture: Maintaining both mammalian and bacterial cell lines, passaging, and cryopreserving. Sound aseptic practices and ability to maintain highly sterile standards.
ü Molecular Biology: Transformation of E. coli, transfection of mammalian cells, and selection of stable transfection, primer designing, ligation, PCR, cloning of gene of interest, Gel electrophoresis.
ü Bioreactors: Setting up bioreactors for mammalian cell growth, maintaining and controlling parameters, and harvesting.
ü Analytical: Experience in handling UV Spectroscopy, Fluorescence Spectroscopy, Fluorescence Microscope, HPLC.
ü Immunological Assays: Performed ELISA, immuno-blotting and immunno-diffusions as part of curriculum.
ü Lab Maintenance: Responsible for the maintaining the lab, organizing the inventory and ordering consumables, documenting cell line information, cleaning incubators, biosafety cabinet.
ü Computer: Minitab and JMP for DOE (Design of experiments), Docking, Rasmol, Pymol, Sequest, Phylip, Spdb Viewer, Microsoft Office, End Note, CAED, Adobe Photoshop.
